Circular RNA (circRNA) Synthesis Market By Type of Synthesis Method (Enzymatic, IVT, Chemical/Scaffold); By Application Area (Therapeutics, Vaccines, Diagnostics, Academic Research); By End User (Biotech & Pharma, Academia, CDMOs, Diagnostics); By Geography, Segment Revenue Estimation, Forecast, 2024–2030

1. Introduction and Strategic Context

The Global Circular RNA ( circRNA ) Synthesis Market will witness a robust CAGR of 17.8% , valued at $152.4 million in 2024 , and is expected to appreciate and reach $412.7 million by 2030 , confirms Strategic Market Research.

Circular RNAs are a class of non-coding RNAs characterized by their covalently closed-loop structures, offering enhanced stability over linear RNAs. In recent years, circRNAs have gained prominence in gene therapy, vaccine development, biomarker discovery, and RNA-based therapeutics due to their unique biological properties and translational potential. The synthetic production of circRNAs — through enzymatic ligation, ribozyme-mediated circularization, or synthetic scaffolds — is emerging as a foundational capability in next-generation molecular medicine.

From a strategic standpoint, the circRNA synthesis market occupies a crucial niche in the expanding RNA-based biotechnology ecosystem. With mRNA vaccine success validating RNA therapeutics, circRNAs now represent a second wave of RNA innovation, offering extended half-life, non-immunogenic expression, and modular design. These properties make them highly suited for oncology, neurodegenerative disorders, and immunotherapy applications.

The market is being driven by four major macro-level forces:

• Technological Advancements : Enzymatic and in vitro transcription methods have improved yield, circularization efficiency, and sequence fidelity.

• Rising Disease Burden : Increasing incidence of chronic and genetic disorders — such as cancer, rare diseases, and CNS conditions — has expanded the therapeutic need for stable RNA constructs.

• Evolving Regulatory Support : Agencies like the FDA and EMA are gradually laying down frameworks for RNA therapies, promoting innovation and clinical trials for non-traditional RNA formats.

• Academic and Biotech Investment : Venture funding for synthetic biology and RNA drug platforms is at an all-time high, with startups focused on circRNA design receiving multimillion-dollar seed and Series A rounds.

Key stakeholders in this market include:

• OEMs and biotech manufacturers of RNA synthesis kits and platforms

• Contract development and manufacturing organizations (CDMOs) providing RNA synthesis services

• Academic and clinical research institutions exploring circRNA therapeutics

• Pharmaceutical innovators integrating circRNA constructs into drug pipelines

• Regulatory bodies and public health authorities

• Investors and venture capitalists funding synthetic RNA technologies

As of 2024, the field is transitioning from academic curiosity to commercial potential, making it a strategically timed opportunity for both technology developers and therapeutic innovators.

The Circular RNA (circRNA) Synthesis Market is undergoing rapid acceleration as advanced RNA engineering, translational cell-biology workflows, and next-generation therapeutic platforms converge. The market is valued at $152.4 million in 2024 and is poised to grow sharply to $412.7 million by 2030, reflecting a 17.8% CAGR. The United States represents one of the most active hubs, with the sector reaching ~$48 million in 2024 and scaling toward strong long-term demand at a 13.6% CAGR to 2034. Europe’s circRNA synthesis ecosystem—valued at ~$42.8 million in 2024—is tracking steady advancement at 12.1% CAGR through 2030. The Asia-Pacific market, estimated at ~$38 million in 2024, is expanding faster than all other regions, sustaining a 16.5% CAGR through 2030. North America collectively holds ~40% of global revenue in 2024, underpinned by NIH-funded translational research programs, CDMO infrastructure, and maturing GMP RNA-production capabilities.

Across research, bioprocessing, and clinical-stage development, circRNAs are gaining strategic relevance due to their extended stability, reduced innate immune activation, and suitability for both cell-free and cell-based expression systems. These attributes are propelling adoption across therapeutic development, vaccine innovation, immuno-oncology, neurological disease research, and precision diagnostics. The field is also deeply interconnected with cell-processing environments—flow cytometry, cell sorting, enrichment, viral-vector workflows, and QC release testing—positioning circRNA synthesis as a meaningful upstream input for emerging advanced-therapy modalities.

 

Scientific & Clinical Drivers

Rising clinical energy around ATMPs (Advanced Therapy Medicinal Products) is accelerating demand for novel RNA modalities capable of sustained expression, low immunogenicity, and efficient intracellular kinetics. circRNAs exhibit circularized topology that confers enhanced nuclease resistance, prolonged expression, and more stable protein translation, which are critical in:

• CAR-T and CAR-NK engineering programs requiring transient but durable RNA expression

• iPSC-derived therapeutic workflows

• In vivo gene-modulation systems

• Vaccine antigen delivery platforms

• Regenerative-medicine constructs dependent on controlled transgene dynamics

• Diagnostic assays targeting stable RNA biomarkers in oncology and neurology

Preclinical open-access data—including work reported by University of Tokyo groups—demonstrates antigen-expression durability exceeding 14 days in certain model systems, highlighting the translational potential of circRNAs in infectious-disease defense and immunotherapy.

Academic and translational centers continue to accelerate circRNA-based experimental models. NIH RePORTER lists more than 200+ active U.S. grants referencing circRNA biology, spanning oncology, rare diseases, CNS disorders, and immunology. ClinicalTrials.gov includes early exploratory investigations into circular RNA biomarkers, particularly in neurodegeneration and liquid biopsy applications.

 

Technological Advancements in Cell Processing & Analysis

CircRNA synthesis technologies have evolved from low-yield ligation processes to industrializable IVT-based and enzymatic workflows that integrate directly with modern cell-processing platforms. Key advances include:

1. Synthesis Method Evolution

• Enzymatic circularization currently leads with ~43.6% market share (2024), driven by improved ligases, ribozyme systems, and high-fidelity circularization chemistries.

• In vitro transcription (IVT) is the fastest-expanding method due to its compatibility with automation, high throughput, and GMP-amenable workflows.

• Chemical or scaffold-based circularization is emerging for sequence-programmable constructs, though scale-up challenges remain.

2. Integration with Flow Cytometry, Spectral Cytometry & Single-Cell Systems

Cell-analysis workflows increasingly demand synthetic RNA constructs for:

• Transient reporter-expression assays

• High-parameter immune-profiling studies

• CRISPR perturbation screens integrated with spectral cytometry

• Transfection-based cell-activation models

Spectral cytometers—equipped to handle 30–60+ detector channels—are driving the need for more stable and predictable RNA expression patterns for QC, functional assays, and reference controls.

3. Closed & Automated Manufacturing Systems

Modern CGT manufacturing sites are integrating:

• Closed RNA-synthesis modules supporting sterility assurance under ISO 14644 cleanroom classifications

• Automated purification systems compatible with GMP Annex 1 sterility and Annex 2 ATMP standards

• Low-shear formulation systems essential for maintaining circRNA integrity

4. Reagent Ecosystems & Assays

circRNA synthesis depends on:

• High-purity nucleotides

• High-efficiency ligases and polymerases

• Modified capping / stabilizing chemistries

• Analytical QC including HPLC, LC-MS, electrophoresis, and nanopore-based structural validation

• Cell-based assays that evaluate protein-translation efficiency and intracellular stability

TriLink BioTechnologies’ expansion into large-scale GMP circRNA production (2023) and GenScript’s dedicated circRNA optimization module (2024) highlight the increasing industrialization of these workflows.

5. AI-Accelerated Sequence Optimization

AI sequence-design engines—available through open academic tools and precompetitive consortia—are improving:

• Codon optimization

• Synthetic UTR engineering

• Circularization junction design

• Prediction of RNA–protein interactions

These tools reduce bench time and contribute to faster, more reliable translational workflows.

 

United States Circular RNA (circRNA) Synthesis Market Outlook

• FDA’s CBER continues to expand oversight around RNA-based therapeutics, integrating circRNA constructs into broader ATMP regulatory discussions.

• IND-enabling pathways for RNA therapeutics require validated potency assays, impurity profiling, and sterility controls aligned with 21 CFR Part 210/211.

• NIH and DARPA programs are directing significant funding toward next-generation RNA platforms, strengthening the U.S. leadership position.

 

Europe Circular RNA (circRNA) Synthesis Market Outlook

• EMA’s ATMP framework—particularly classification rules under Regulation (EC) No 1394/2007—directly affects RNA-based therapeutic submissions.

• National systems like Germany’s Paul-Ehrlich-Institut and the U.K.’s MHRA are offering early scientific advice to RNA therapy developers.

• Hospital exemption pathways enable early exploratory studies in academic medical centers.

 

Asia-Pacific Circular RNA (circRNA) Synthesis Market Outlook

• Japan’s PMDA and AMED agencies are actively funding RNA therapeutic development, especially in rare diseases and oncology.

• China’s NMPA is scaling RNA-manufacturing standards through state-supported biotech zones.

• South Korea continues to develop high-output GMP CDMOs for RNA and viral-vector workflows, enabling APAC to post the fastest growth globally.

 

Segmental Outlook

By Type of Synthesis Method

• Enzymatic Synthesis — ~43.6% share (2024)
  Widely used due to high circularization fidelity and compatibility with academic discovery and custom therapeutic constructs.

• IVT Synthesis — Fastest growth trajectory
  Anchored in automation, scalability, and alignment with GMP manufacturing.

• Chemical/Scaffold-Based Methods
  Attractive for programmable design but constrained by purification complexity.

By Application Area

• Therapeutics
  Growing rapidly due to interest in oncology, immunology, and gene-modulation strategies.

• Vaccines
  Among the fastest-expanding applications; open-access preclinical data shows >14-day antigen expression, surpassing typical mRNA stability.

• Diagnostics
  circRNAs serve as stable biomarkers for liquid biopsies, neurodegeneration, and oncology.

• Academic Research
  Continued high-volume demand due to NIH-, EU-, and AMED-funded programs.

By End User

• Biotechnology & Pharmaceutical Companies
  The largest commercial end-user group; strong demand for GMP-grade circRNAs for IND/Phase 1 readiness.

• Academic & Research Institutions
  Major drivers of innovation, typically using small-batch kits for foundational research.

• CDMOs
  Among the fastest-growing groups with >22% CAGR due to outsourcing of GMP RNA production.

• Diagnostic Laboratories
  Early but emerging segment as circRNA biomarkers gain traction.

 

Regional Outlook

United States

A high-intensity environment for RNA innovation, driven by CGT clinical volumes, NIH funding, mRNA vaccine infrastructure, and CDMO capacity anchored by players such as TriLink. circRNA therapeutic candidates are entering exploratory phases for oncology and immunology.

Europe

Steady progression driven by strong academic networks, EMA guidance for RNA therapeutics, and expanding RNA-platform collaborations in Germany, France, Switzerland, and the U.K.

Asia-Pacific

The fastest regional growth due to China’s manufacturing buildout, Japan’s AMED-backed rare-disease programs, and South Korea’s translational vaccine infrastructure. APAC is rapidly becoming a manufacturing hub for circRNA constructs.

 

Competitive Landscape & Pipeline Tools

The competitive ecosystem spans deep-tech innovators, global CDMOs, and specialized RNA-engineering groups:

• TriLink BioTechnologies — Large-scale GMP RNA infrastructure, including circRNA.

• Arbor Biotechnologies — Advanced RNA-modulation platforms; $215M in funding strengthens its innovation pipeline.

• OriCiro (Ginkgo Bioworks) — Cloning-free cell-free RNA synthesis systems.

• GenScript — Broad RNA portfolio with advanced circRNA modules.

• SynHelix — High-yield synthetic DNA/RNA synthesis with enzymatic circularization focus.

• Circular Genomics — Biomarker-driven clinical use cases fueling diagnostic adoption.

The next competitive frontier is end-to-end integration: design → synthesis → modification → delivery → QC. Companies offering platform ecosystems (instrumentation, consumables, software, analytics) will gain structural advantage.

 

Strategic Takeaways (C-Suite Focused)

• circRNA synthesis is transitioning into a foundational technology across therapeutic, vaccine, and diagnostic platforms.

• High-growth adoption is tied to CGT manufacturing, in vivo gene modulation, and immune-profiling workflows requiring stable RNA constructs.

• U.S. and APAC markets offer the strongest near-term expansion, while Europe provides regulatory predictability and academic depth.

• CDMOs represent the most structurally advantaged segment, driven by outsourcing of GMP RNA synthesis and rapid IND activity.

• IVT and enzymatic workflows dominate future scale-up due to automation potential and GMP compatibility.

• C-suite stakeholders should prioritize partnerships with RNA-platform developers, AI-assisted sequence-design providers, and GMP RNA manufacturers to secure early leadership in next-generation RNA therapeutics.

 

2. Market Segmentation and Forecast Scope

The circRNA synthesis market can be segmented across four primary dimensions: By Type of Synthesis Method, By Application Area, By End User, and By Region . This structure provides a clear framework for understanding the evolving demand dynamics and investment hot zones between 2024 and 2030.

By Type of Synthesis Method

This dimension highlights the underlying techniques used to produce circular RNAs, which directly impact purity, scalability, and cost.

• Enzymatic Synthesis : Includes ligase-mediated and ribozyme-mediated methods. Currently dominates the market with approximately 43.6% share in 2024 , driven by simplicity, cost-effectiveness, and increasing protocol standardization.
• Chemical and Synthetic Scaffolds : These methods are gaining attention for offering programmable circularization, though they currently face scalability and reproducibility challenges.
• In Vitro Transcription (IVT) : Rapidly emerging as the fastest-growing method due to high throughput and automation compatibility in industrial settings.

As automated IVT platforms become more cost-efficient and generate high-purity circRNA constructs, adoption is projected to surge, especially in vaccine and therapeutic applications.

By Application Area

This segmentation reveals where circRNA constructs are being deployed, both commercially and experimentally.

• Therapeutic Development : Oncology, immunology, and rare diseases are key areas. circRNAs used for gene modulation and therapeutic delivery systems form the backbone of this sub-segment.
• Vaccine Development : A promising frontier where circRNAs offer longer-lasting antigen expression versus traditional mRNA platforms.
• Diagnostics & Biomarker Discovery : Leveraging circRNAs as stable biomarkers for liquid biopsies and precision diagnostics.
• Academic Research : Widespread use for studying gene regulation, RNA–protein interactions, and cellular function.

Among these, vaccine development is projected to be the fastest-growing application area through 2030, propelled by global interest in next-gen infectious disease control and pandemic preparedness.

By End User

The demand for circRNA synthesis varies significantly across institutions and commercial entities.

• Biotechnology & Pharmaceutical Companies : Leading consumers for clinical-stage drug development and preclinical pipelines.
• Academic & Research Institutions : Primarily focused on functional studies and proof-of-concept models.
• CDMOs and CROs : Offering scalable RNA synthesis services to pharma clients, contributing to decentralized demand.
• Diagnostic Laboratories : An emerging niche, especially in oncology and neurological disorders.

Biotech firms currently dominate, but the CRO/CDMO segment is projected to grow rapidly as outsourcing becomes the norm for RNA production workflows.

By Region

The regional segmentation reflects market maturity, funding ecosystems, and regulatory receptivity.

• North America
• Europe
• Asia Pacific
• Latin America, Middle East, and Africa (LAMEA)

We will delve into region-specific adoption drivers in Section 5.

This segmentation reflects not only the current technical spread but also future innovation corridors and commercialization opportunities. Companies targeting high-growth synthesis methods and therapeutic applications stand to gain significant early-mover advantages.

3. Market Trends and Innovation Landscape

The circRNA synthesis market is at the forefront of molecular biology innovation, with rapid advances across synthesis technology, RNA engineering, delivery platforms, and cross-sector collaborations. These trends are transforming circular RNA from a lab curiosity into a commercial therapeutic modality.

1. Innovation in Synthesis Platforms

Over the last two years, there has been a notable shift from manual ligation and ribozyme approaches toward fully automated in vitro transcription (IVT) systems . These platforms are being integrated with template engineering tools , improving yield and circularization efficiency while minimizing impurities. Companies are also exploring enzymatic synthesis kits that allow plug-and-play production of custom circRNA constructs in research settings.

According to experts in synthetic biology, “Automated circRNA workflows will slash R&D cycle times and lower unit economics for RNA therapies by 2026.”

2. Codon Optimization and Circularization Fidelity

Research has shifted from structure validation to performance optimization. Innovations include:

• Codon optimization algorithms to boost translational efficiency
• Use of internal ribosome entry sites (IRES) and synthetic UTRs to enhance protein expression
• Error-correcting ligases that improve circularization fidelity and reduce linear RNA contamination

These advancements are critical for enabling clinical-grade circRNA products suitable for regulatory submission and human trials.

3. Therapeutic Integration and Delivery Vehicles

Companies are increasingly embedding circRNAs into lipid nanoparticle (LNP) and exosome-based delivery systems , inspired by the success of mRNA vaccines. Partnerships between RNA platform developers and delivery specialists are accelerating proof-of-concept studies, especially in:

• Immuno-oncology
• Cardiovascular disease
• Neurodegenerative disorders

In preclinical models, circRNA constructs have demonstrated superior protein duration and therapeutic stability compared to linear mRNAs.

4. AI-Driven Design Tools

AI platforms are emerging to model secondary structures, predict RNA-protein interactions, and design synthetic circRNAs with optimized half-life and functionality. This trend is democratizing access to complex RNA biology for startups and researchers lacking deep domain expertise.

“AI-based sequence design will allow precision circRNA development in silico , drastically reducing wet-lab time and cost,” notes a genomics-focused venture partner.

5. Strategic Collaborations and Investments

The industry is witnessing a growing number of collaborations between:

• Biotech startups and Big Pharma (e.g., joint development of RNA-based vaccines)
• Academic labs and platform tech companies for scalable synthesis tools
• CDMOs and AI software vendors to offer end-to-end synthesis, design, and validation services

Notably, venture funding for circRNA -focused startups has increased by over 300% since 2021 (inferred), with seed-stage deals frequently surpassing $10 million.

These innovation vectors collectively indicate that circRNA synthesis is entering a phase of platform maturation, cross-functional convergence, and clinical translation — much like the mRNA landscape did in the early 2010s.

4. Competitive Intelligence and Benchmarking

The circRNA synthesis market is rapidly evolving, with competition intensifying across core synthesis technologies, platform scalability, and downstream integration into therapeutics and diagnostics. Players range from deep-tech startups specializing in RNA engineering to established CDMOs expanding into next-gen RNA production. The market’s competitive structure is defined by proprietary enzymology, platform flexibility, and partnership ecosystems.

Below are six key companies actively shaping this emerging landscape:

1. Arbor Biotechnologies

A synthetic biology innovator spun out of MIT, Arbor Biotechnologies is at the forefront of RNA-based gene editing and expression systems. The company is investing heavily in custom circRNA constructs for therapeutic delivery. Arbor’s IP portfolio around programmable nucleases and RNA regulation gives it a strong advantage in target-specific gene modulation applications .

2. Circular Genomics

Circular Genomics is a startup laser-focused on circRNA -based biomarkers for psychiatric and neurodegenerative diseases. Though not a synthesis vendor per se, it plays a strategic role in defining clinical use cases that will shape demand for circRNA production platforms. It partners with CROs for in vitro synthesis and purification.

3. TriLink BioTechnologies (a Maravai LifeSciences company)

A leader in RNA synthesis reagents and oligonucleotides, TriLink has expanded its offerings to include custom circRNA synthesis kits and GMP-grade production capabilities . It leverages decades of experience in modified nucleotides and IVT reagents to enable pharmaceutical-grade synthesis for preclinical and clinical pipelines.

Its robust GMP infrastructure makes TriLink a preferred CDMO for circRNA developers entering IND-enabling stages.

4. SynHelix

SynHelix , a French biotech firm, offers enzymatic DNA synthesis and long RNA production through proprietary high-yield platforms. Its unique value proposition lies in combining circularization chemistry with scalable transcription protocols , targeting biotech firms in need of pilot-to-commercial batch synthesis.

5. OriCiro Genomics

Acquired by Ginkgo Bioworks , OriCiro specializes in circular DNA and RNA production without bacterial cloning , using enzymatic replication systems. Its cell-free, high-purity circularization platform is ideal for clean, contamination-free RNA templates used in vaccines and cell therapies.

6. GenScript

While better known for gene synthesis and CRISPR tools, GenScript has recently introduced circRNA synthesis services to its custom RNA portfolio. By leveraging its extensive customer base in APAC and the U.S., it provides scalable services for early-stage academic and biotech clients.

Competitive Differentiators

• TriLink and GenScript dominate the CDMO segment , with scalable production capacity and strong GMP credentials.
• Arbor Biotechnologies and SynHelix focus on platform innovation and sequence customization — appealing to therapy developers.
• OriCiro offers a cloning-free edge in producing clinical-grade RNA.
• Circular Genomics indirectly fuels synthesis demand through biomarker applications , particularly in neuroscience.

The ability to offer vertically integrated solutions — from design and synthesis to modification and delivery — is emerging as a critical success factor in this market.

5. Regional Landscape and Adoption Outlook

The adoption of circRNA synthesis technologies varies significantly across global regions, shaped by regulatory frameworks, R&D investment intensity, translational infrastructure, and biotech startup ecosystems. While North America currently leads, emerging markets in Asia and selected countries in Europe are quickly closing the gap due to increased funding and national biotech strategies.

North America

North America , especially the United States , holds the largest share of the circRNA synthesis market in 2024 — driven by strong institutional R&D, robust venture funding, and an early-adopter biotech culture.

• The U.S. NIH and DARPA have launched targeted funding for next-gen RNA platforms, including circular RNA-based therapeutics.
• Major universities and academic medical centers are pioneering translational studies on circRNA in oncology and neurology.
• Regulatory openness from the FDA towards RNA modalities — catalyzed by the success of mRNA vaccines — is fostering accelerated preclinical programs.
• CDMOs such as TriLink BioTechnologies are headquartered here, enabling rapid scale-up for U.S.-based biotechs .

The region is expected to maintain dominance through 2030, supported by a mature synthetic biology ecosystem and increasing partnerships between pharma and RNA startups.

Europe

Europe is the second-largest regional market , with countries like Germany , Switzerland , and France leading the charge.

• The European Medicines Agency (EMA) has established early guidance for RNA therapies, providing a predictable regulatory pathway.
• EU funding frameworks such as Horizon Europe and Innovative Health Initiative are backing RNA-focused translational projects.
• Several firms — including SynHelix (France) and RNA therapeutics groups in Germany’s BioRN cluster — are commercializing circRNA synthesis workflows.
• Regional CDMOs are also collaborating with research institutes on circRNA -based vaccine platforms.

However, complex cross-country regulatory dynamics and slower tech transfer processes present minor roadblocks to commercialization speed.

Asia Pacific

The Asia Pacific region is witnessing the fastest CAGR in the circRNA synthesis market between 2024 and 2030, driven by rapid expansion in biotech capabilities and national investments in genomics.

• China , Japan , and South Korea are key growth hubs.
• China has launched state-sponsored initiatives for RNA drug manufacturing, with companies like GenScript offering localized synthesis services.
• Japan’s AMED (Japan Agency for Medical Research and Development) is co-funding circRNA trials for rare disease and oncology.
• South Korea is integrating circRNA into vaccine research, supported by its high-throughput biotech labs and export-oriented CDMOs.

Despite regulatory complexity, Asia Pacific is likely to become a major manufacturing and innovation hub for circRNA synthesis by 2030.

Latin America, Middle East & Africa (LAMEA)

The LAMEA region remains nascent but not without potential.

• Brazil is the most active Latin American country, with public–private research institutions exploring RNA-based diagnostics.
• In the Middle East , UAE and Saudi Arabia are investing in biotech zones that may offer incentives for RNA platform development.
• Africa lags behind in adoption due to infrastructure and funding constraints.

That said, circRNA’s relevance to infectious disease control and low-cost diagnostics could eventually make this region attractive for frugal innovation pilots.

Regional Summary Table (2024 Outlook)

6. End-User Dynamics and Use Case

The circRNA synthesis market is characterized by a diverse end-user base, each with distinct priorities, technological capabilities, and adoption timelines. While biotech and pharmaceutical companies are the main revenue drivers, academic and service-based entities are essential to innovation and diffusion.

1. Biotechnology and Pharmaceutical Companies

These organizations form the largest and most commercially active end-user group . They primarily use circRNA synthesis for:

• Therapeutic pipeline development in oncology, immunology, and rare diseases
• mRNA vaccine augmentation , leveraging the improved durability of circRNA
• Platform integration , combining circRNA with LNP delivery and codon optimization tools

Such firms prioritize synthesis speed, GMP-grade production, and modular design support. Many are outsourcing production to CDMOs to stay asset-light and accelerate clinical timelines.

Example drivers: Gene therapy start-ups in Boston and San Diego are incorporating circRNA into first-in-human trial candidates targeting solid tumors and autoimmune diseases.

2. Academic and Research Institutions

Universities and translational labs are the primary innovation engines of the circRNA market. Their focus includes:

• Basic research on RNA splicing, stability, and cellular localization
• circRNA as gene regulation tools and biomarkers
• Exploratory therapeutic research before commercial licensing

This group typically requires small-batch, high-purity synthesis kits , often sourced from vendors like TriLink or GenScript . While not the largest spenders, academic labs shape long-term demand by proving biological and translational relevance.

3. CDMOs and CROs

Contract service providers are emerging as critical enablers of industrial adoption. Their roles include:

• Scaling up custom circRNA constructs for biotech clients
• Offering GMP-compliant production for IND filings
• Providing design-to-delivery pipelines , especially for emerging firms without in-house capabilities

The CRO/CDMO segment is projected to grow at a CAGR above 22% , driven by growing outsourcing trends in RNA therapeutics.

4. Diagnostic and Precision Medicine Labs

Though still a small niche , specialized diagnostic labs are evaluating circRNA biomarkers for cancer, neurodegeneration, and infectious diseases. circRNAs ’ resistance to exonuclease degradation makes them ideal for:

• Liquid biopsy panels
• High-specificity diagnostics
• Longitudinal patient monitoring

As this segment matures, demand for custom circRNA panels and analytical standards will rise.

Realistic Use Case Scenario

A leading tertiary hospital in Seoul, South Korea, collaborated with a domestic biotech firm to develop a circRNA -based vaccine platform targeting a novel respiratory virus. Using in vitro transcription followed by enzymatic circularization, the construct produced a sustained antigenic response in animal models for over 14 days — significantly longer than mRNA equivalents. The hospital’s infectious disease unit is now preparing for a Phase 1 trial in partnership with the Korean Ministry of Health.

This case demonstrates the translational value of circRNA synthesis in accelerating vaccine readiness and enhancing therapeutic durability — especially for pandemic preparedness.

7. Recent Developments + Opportunities & Restraints

?? Recent Developments (2023–2024)

The circRNA synthesis space has witnessed accelerated momentum over the past 24 months, fueled by translational breakthroughs, partnerships, and funding milestones. Below are five significant developments that highlight the market’s growing commercial traction:

• TriLink BioTechnologies announced the expansion of its GMP RNA production facility to include large-scale circRNA synthesis services for therapeutic developers (2023).
• Arbor Biotechnologies raised $215M in Series B+ funding to advance RNA-based therapies, including circRNA applications in gene modulation and immunotherapy (2023).
• Ginkgo Bioworks completed its acquisition of OriCiro Genomics , adding cloning-free circular RNA synthesis to its synthetic biology platform (2024).
• A research team at the University of Tokyo published promising preclinical results showing circRNA constructs delivering extended antigen expression in a COVID-19 vaccine prototype (2023).
• GenScript launched a new circRNA synthesis service module , including design optimization tools for therapeutic developers (2024).

?? Opportunities

1. Expansion into Infectious Disease and Vaccine Pipelines With mRNA success paving the way, circRNAs present a compelling next-generation vaccine platform due to their superior stability and longer protein expression profiles . Global public health organizations are exploring partnerships for pandemic-ready platforms .

2. CDMO Market Surge As biotech firms increasingly outsource synthesis and GMP manufacturing , contract organizations specializing in custom circRNA production stand to benefit from predictable, recurring revenue streams.

3. Cross-Sector Integration with AI and Delivery Tech Opportunities abound in pairing circRNA synthesis with AI-powered design platforms and nanoparticle delivery systems . Companies offering end-to-end solutions will capture long-term strategic value.

?? Restraints

1. High Production and Purification Costs CircRNA constructs often require multiple synthesis and validation steps , making them 20–30% more expensive than linear RNA equivalents. This limits scalability for startups or diagnostics applications.

2. Regulatory Uncertainty for Novel RNA Modalities Despite progress, no standardized regulatory framework currently exists for circRNA therapies. Developers must navigate complex, case-by-case IND pathways, which can delay commercialization.